The subject invention concerns materials and methods for alkene reduction of compounds, such as levoglucosenone (LGO) and (S)-γ-hydroxymethyl-α,β-butenolide (HBO), using an alkene reductase enzyme. In one embodiment, a method of the invention comprises alkene reduction of a target compound by reacting the compound with an Old Yellow Enzyme (OYE) that reduces alkene bonds. In one embodiment, the OYE is OYE 2.6 from Pichia stipites and comprises the amino acid sequence of SEQ ID NO:1.
In a specific embodiment, the enzyme is an Old Yellow Enzyme (OYE) 2.6 mutant having an amino acid substitution at position Q 78 in the sequence, wherein the tyrosine at position 78 is substituted with a tryptophan amino acid (Y78W) and is designated as OYE 2.6 Y78W (SEQ ID NO:2).
The invention concerns a bee smoker characterised in that it comprises an enclosure inside which there is arranged an interchangeable cartridge containing a non-glycol active composition, in liquid phase that can be volatilised, said enclosure opening into a smoking duct and having an inlet for a pulsed air flow produced by an air flow generator the bee smoker comprising a means for vaporising said active composition without the combustion of said composition. The invention also concerns an active composition intended for smoking bees containing at least 15% triethyl citrate.
The invention relates to a method for converting levoglucosenone into 4-hydroxymethyl butyrolactone and 4-hydroxymethyl butenolide without using any solvent or catalyst, wherein the oxidation and hydrolysis of the levoglucosenone or hydrogenated levoglucosenone are carried out in a single step using an aqueous solution of H2O2.
The present invention relates to a method for preparing a precursor of a single dairy-lactone isomer, to a single dairy-lactone isomer, and to the organoleptic uses thereof.
Our second patent has been lodged! This patent is related to the transformation of levoglucosenone into platform chemicals. These molecules are intended to be building blocks for food/feed, polymers and pharmaceutical industries. If you are interested in this technology please contact our business development team for further information.
Our first patent approval rewards the tremendous work of our team on lignin (bio)transformation. It is related to the preparation and utilisation of polyphenols with exclusively biaryl bonds. Obtained through biocatalysed oligomerisation of 100% renewable monomers, these polyphenols exhibit antioxidant, antiradical and antimicrobial properties. Furthermore, these polymers are good plasticizers, allowing them to be considered as attractive bisphenol A substitutes.
These polyphenols are 100% bio-based and non toxic, as a consequence, they are suitable for applications in the food and cosmetic industries. If you want to know more about this patent and possible applications please contact our business development team.