The invention relates to the field of producing 4-hydroxymethyl-γ-butyrolactone (2H-HBO) from levoglucosenone (LGO). More particularly, the invention concerns an eco-compatible method for synthesising 2H-HBO optionally substituted at the β-position of the lactone function from LGO or a saturated form of LGO such as dihydrolevoglucosenone (2H-LGO) or LGO hydrate (OH-LGO) via a biocatalytic reaction using a cyclohexanone monooxygenase (CHMO).
The invention relates to biobased anti-UV molecules. In particular, the invention relates to a novel process for preparing b-b dimers having anti-UV and antioxidant properties, from p-hydroxycinnamic esters and amides disubstituted in the ortho position with respect to the phenol function and from ketones disubstituted in the ortho position with respect to the phenol, in particular from sinapic acid esters and amides and ketone analogues. The dimers of formulae (I), (II), (III) and (IV) as obtained by means of the process according to the invention can be used for the production of polymers/plastics (in plastics technology), for the protection of plants against the cold and as cosmetic or food-processing ingredients.
The subject invention concerns materials and methods for alkene reduction of compounds, such as levoglucosenone (LGO) and (S)-γ-hydroxymethyl-α,β-butenolide (HBO), using an alkene reductase enzyme. In one embodiment, a method of the invention comprises alkene reduction of a target compound by reacting the compound with an Old Yellow Enzyme (OYE) that reduces alkene bonds. In one embodiment, the OYE is OYE 2.6 from Pichia stipites and comprises the amino acid sequence of SEQ ID NO:1.
In a specific embodiment, the enzyme is an Old Yellow Enzyme (OYE) 2.6 mutant having an amino acid substitution at position Q 78 in the sequence, wherein the tyrosine at position 78 is substituted with a tryptophan amino acid (Y78W) and is designated as OYE 2.6 Y78W (SEQ ID NO:2).
The invention concerns a bee smoker characterised in that it comprises an enclosure inside which there is arranged an interchangeable cartridge containing a non-glycol active composition, in liquid phase that can be volatilised, said enclosure opening into a smoking duct and having an inlet for a pulsed air flow produced by an air flow generator the bee smoker comprising a means for vaporising said active composition without the combustion of said composition. The invention also concerns an active composition intended for smoking bees containing at least 15% triethyl citrate.
The invention relates to a method for converting levoglucosenone into 4-hydroxymethyl butyrolactone and 4-hydroxymethyl butenolide without using any solvent or catalyst, wherein the oxidation and hydrolysis of the levoglucosenone or hydrogenated levoglucosenone are carried out in a single step using an aqueous solution of H2O2.
The present invention relates to a method for preparing a precursor of a single dairy-lactone isomer, to a single dairy-lactone isomer, and to the organoleptic uses thereof.